Tracer Techniques for the Study of Host-parasite Relations
نویسنده
چکیده
TRACER TECHNIQUES FOR THE STUDY OF HOST-PARASITE RELATIONS. Autoradiographic techniques have been used to study the interaction of many facultative and obligate parasites, including viruses. After feeding the host plant with labelled substrates, labelled material accumulates in the infected cells and seems to penetrate into structures of the parasite. After labelling the parasite, its influence on the host may be studied. We use this technique to study the interaction of host (bean) and parasite (bean rust) during the infection process. After infection with uredospores labelled with tritiated orotic acid, the radioactivity is retained almost completely within the young haustorium at 22 h after inoculation. This may indicate a very small influence of the parasite on its compatible host. In incompatible host-parasite combinations, the infection process proceeds in a different way. The use of autoradiographic techniques to compare combinations of varying compatibilities will be discussed.
منابع مشابه
Isolation and Purification of the Schizont Stage of Theileria annulata from Host Leukocytes through Novel Biochemical Techniques
The intracellular protozoan parasite, Theileria annulata, induces uncontrolled proliferation and transformation in bovine B lymphocytes and monocytes in blood circulation andlymph nodes of host cells. This uncontrolled replication happens in the macroschizont stage of the life cycle of the parasites. The development of a rapid and efficient technique is likely to necessita...
متن کاملArginase Activity and Its Effects on Pathogenesis of Leishmania
Leishmaniasis is a tropical parasitic disease that has become a major health challenge in many countries of the world. Not only has not been found any effective vaccine or treatment for the disease eradication, but also the advent of drug resistance is also increasing. Therefore, it is vital to take a precise attention to the physiochemical cycles of the Leishmania parasite and to identify i...
متن کاملژنومیکس انگل ها
Genes carry instructions to make protein that affect body's cells and their physical activity. They also play an important role in the occurrence of various characteristics in the body. Recently, scientists in the new field of science known as genomics have studied the genetic instructions. Genomics deals with the discovery of all the sequences in the entire genome of organisms and is used to s...
متن کاملSerological Evaluation of Experimental Toxoplasma gondii Infection in Cats by Using Immunoblotting Based on an Affinity Purified Surface Antigen
Toxoplasma gondii is an apicomplexan parasite that infects human and almost all warm-blooded animals. The life cycle of the parasite includes an asexual reproduction in intermediate hosts (Mammals and birds) and a sexual reproduction in definitive hosts (Felidae). Cats are both the intermediate and the definitive host for T. gondii. The aim of this study was to investigate anti-T. gondii antibo...
متن کامل